Background:
@EN Non-insulin-dependent diabetes mellitus(NIDDM) is postulated to be associated with increased lipid peroxidation, which may contribute to vascular and other complications of the syndrome. Such oxidative stress has, however, been difficult to
assess
because specific and reproducible methods for the measurement of products of lipid peroxidation have been unavailable, especially in plasma.
@ES Methods:
@EN To see whether NIDDM patients show increased plasma lipid peroxidation, plasma lipid hydroperoxides were measured by the ferrous oxidation with xylenol orange (FOX II) assay coupled with the selective lipid hydroperoxide reductant
triphenylphosphine(TPP). Formation of the ferricxy lenol orange complex was determined at 560 nm and calibrated against H2O2.
@ES Results :
@EN For 60 patients with NIDDM, a concentration of 4.26±2.92μmol/l(mean±SD) lipid hydroperoxides was recorded. This concentration was higher(<0.001) than that of plasma lipid hydroperoxides from 20 control subjects(1.68±1.65μmol/l). Plasma
lipid
hydroperoxides in the diabetic group did not correlate with ages, durations of diabetes, fasting glucose, HbA10 levels, total plasma cholesterol, TG, and diabetic retinopathy.
@ES Conclusion :
@EN The finding of increased levels of plasma lipid hydroperoxides in NIDDM provides direct evidence that NIDDM is associated with oxidative stress. It is suggest that the increased plasma lipid hydroperoxides in NIDDM be further evaluated for
its
clinical significance and for association with glucose control, lipid metabolism, and vascular complications.
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